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    Structured Review

    Bio-Rad developer
    Developer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+developer/Clarity+Western+ECL+Substrate/bio_rxiv__64898__2025__12__31__697216-147-14-15
    Average 99 stars, based on 15622 article reviews
    developer - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). ..

    Article Title: Yi-Shen-Hua-Shi granules inhibit diabetic nephropathy by ameliorating podocyte injury induced by macrophage-derived exosomes
    Article Snippet: After blocking with 4% milk containing 0.1% Tween, add primary anti-CD9 antibody (1:2000; Abcam, ab92726), CD63 antibody (1:2000; Abcam, ab216130), CD81 antibody (1:1000; Abcam, ab155760), caspase-3 antibody (1:2000; Abcam, ab184787), GAPDH antibody (1:2500; Abcam, ab9485), and incubate overnight at 4°C. .. After adding HRP-labeled secondary antibody (Dianova, Hamburg, Germany) and incubating at room temperature for 2 h, the ECL developer (Bio-Rad no. 170–5060) was added dropwise on the membrane and placed into the Gel Doc imaging system (Bio-Rad) for taking pictures. ..

    Incubation:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). ..

    Labeling:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). ..

    Imaging:

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2.
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). .. The relative protein expression levels were normalized with GAPDH as the internal reference of total protein and Lamin B as the internal reference of nuclear protein. qRT‐PCR Total RNA was extracted from cells using Trizol (Invitrogen life technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA by reverse transcription kit.

    Article Title: Maresin1 alleviates liver ischemia/reperfusion injury by reducing liver macrophage pyroptosis.
    Article Snippet: .. The protein bands were detected using ECL developer and a Bio-Rad imaging system (Bio-Rad, USA). ..

    Article Title: NEK2 affects the ferroptosis sensitivity of gastric cancer cells by regulating the expression of HMOX1 through Keap1/Nrf2
    Article Snippet: The PVDF membrane was incubated at 4 °C overnight with primary antibody (NEK2 antibody, abcam, ab279717; Nrf2 antibody, affnity, BF8017; keap1 antibody, abcam, ab227828; HMOX1 antibody, abcam, ab68477; GAPDH antibody, abcam, ab9485; Lamin B antibody, abcam, ab16048). .. The PVDF membrane was washed with TBST buffer and incubated with the secondary antibodies labeled with HRP (Dianova, Hamburg, Germany) at room temperature for 2 h, and washed with TBST and then photographed with ECL developer (Bio-Rad, USA, no.170-5060) and GelDoc imaging system (Bio-Rad). ..

    Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1
    Article Snippet: .. BioRad ECL developer was added to the blot for 5 min and left on a shaker before imaging of the blot was performed. .. All blots were imaged using a BioRad imaging system.

    Article Title: Yi-Shen-Hua-Shi granules inhibit diabetic nephropathy by ameliorating podocyte injury induced by macrophage-derived exosomes
    Article Snippet: After blocking with 4% milk containing 0.1% Tween, add primary anti-CD9 antibody (1:2000; Abcam, ab92726), CD63 antibody (1:2000; Abcam, ab216130), CD81 antibody (1:1000; Abcam, ab155760), caspase-3 antibody (1:2000; Abcam, ab184787), GAPDH antibody (1:2500; Abcam, ab9485), and incubate overnight at 4°C. .. After adding HRP-labeled secondary antibody (Dianova, Hamburg, Germany) and incubating at room temperature for 2 h, the ECL developer (Bio-Rad no. 170–5060) was added dropwise on the membrane and placed into the Gel Doc imaging system (Bio-Rad) for taking pictures. ..



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